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Author: Kawana, Y.; Komiya, A.; Ueda, T.; Nihei, N.; Kuramochi, H.;
Suzuki, H.; Yatani, R.; Imai, T.; Dong, J. T.; Yoshie, O.; Barrett,
J. C.; Isaacs, J. T.; Shimazaki, J.; Ito, H.; Ichikawa, T.
Year: 1997
Title: Location of KAI1 on the short arm of human chromosome 11 and
frequency of allelic loss in advanced human prostate cancer
Journal: Prostate
Volume: 32
Issue: 3
Pages: 205-13
Label: 97398686
Keywords: Adenocarcinoma/*genetics/pathology
*Alleles
Animal
Antigens, CD/*genetics
Autopsy
Base Sequence
*Chromosome Mapping
*Chromosomes, Human, Pair 11
DNA Primers/analysis/chemistry/genetics
DNA, Neoplasm/analysis/chemistry/genetics
Gene Frequency
Genes, Suppressor, Tumor/*genetics
Heterozygote
Human
Immunohistochemistry
In Situ Hybridization, Fluorescence
Male
Membrane Glycoproteins/*genetics
Neoplasm Staging
Polymerase Chain Reaction
Prostatic Neoplasms/*genetics/pathology
Rats
Support, Non-U.S. Gov't
Tumor Cells, Cultured
Abstract: BACKGROUND: We recently isolated the KAI1 gene, a metastasis
suppressor gene for prostate cancer, from human chromosome region
11p13-cen- containing rat prostate cancer cells. The present study
was performed to further locate the region of the KAI1 gene on
the short arm of chromosome 11, and to examine whether loss of
this region is significant during progression of human prostate
cancer. METHODS: The small portion of human chromosome 11 (i.e.,
11p13-cen) was reintroduced into highly metastatic rat prostate
cancer cells by using microcell- mediated chromosome transfer.
Loss of heterozygosity (LOH) at polymorphic microsatellite loci
on the human chromosome 11 was examined in human prostate cancer
tissues. RESULTS: The minimum region of human chromosome 11 that
contained the KAI1 gene was located on the proximal region of
11p11.2 divided by the D11S554 locus. The percentage of LOH or
allelic imbalance at the D11S1344 locus, which is located on the
same region as the KAI1 locus, in metastasis tissues from autopsy
cases who died from metastatic prostate cancer was 70% (7 of 10
informative cases), whereas the percentages in primary tumors
from the same cases and from cases with clinically localized prostate
cancer were 33% (3 of 9 informative cases) and 8% (1 of 12 informative
cases), respectively. CONCLUSIONS: These findings demonstrate
a high frequency of LOH or allelic imbalance at the centromeric
region of 11p, which contains the KAI1 gene in advanced prostate
cancer.