go to Japanese home page.....
3
Author: Komiya, A.; Suzuki, H.; Aida, S.; Yatani, R.; Shimazaki, J.
Year: 1995
Title: Mutational analysis of CDKN2 (CDK4I/MTS1) gene in tissues and
cell lines of human prostate cancer
Journal: Jpn J Cancer Res
Volume: 86
Issue: 7
Pages: 622-5
Label: 96004844
Keywords: Base Sequence
Carrier Proteins/biosynthesis/*genetics
Cell Line
DNA Mutational Analysis
DNA Primers
Exons
*Genes, Suppressor, Tumor
Human
Japan
Male
Molecular Sequence Data
Neoplasm Staging
*Point Mutation
Polymerase Chain Reaction
*Polymorphism (Genetics)
Prostatic Neoplasms/*genetics/pathology
*Sequence Deletion
Support, Non-U.S. Gov't
Tumor Cells, Cultured
Abstract: To study mutation of the CDKN2 gene in prostate cancer, samples
from 51 Japanese patients and four human prostate cancer cell
lines were examined by single-strand conformation polymorphism
analysis and direct sequencing. Only one out of 51 (2%) patients
revealed a mutation, which was a 24 bp deletion from the 5'-untranslated
region to codon 3, resulting in loss of the initiation site. One
of the four cell lines revealed a missense mutation, a GAC-->TAC
(Asp-->Tyr) at codon 84. These results indicate that mutation
of the CDKN2 gene is rare in prostate cancer and thus does not
contribute significantly to the pathogenesis of human prostate
cancer. Prostate cancer cell lines may acquire more frequent abnormality
of the CDKN2 gene than tumor tissues.