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Author: Komiya, A.; Suzuki, H.; Ueda, T.; Aida, S.; Ito, N.; Shiraishi,
T.; Yatani, R.; Emi, M.; Yasuda, K.; Shimazaki, J.; Ito, H.
Year: 1997
Title: PRLTS gene alterations in human prostate cancer
Journal: Jpn J Cancer Res
Volume: 88
Issue: 4
Pages: 389-93
Label: 97341057
Keywords: Adenocarcinoma/*genetics/pathology
Alleles
Cell Differentiation
Chromosomes, Human, Pair 8
DNA Mutational Analysis
Gene Deletion
*Genes, Suppressor, Tumor/*genetics
Human
Male
Polymorphism, Single-Stranded Conformational
Prostatic Neoplasms/*genetics/pathology
Support, Non-U.S. Gov't
Abstract: Since loss of heterozygosity on 8p22-p21.3 has been found frequently
in prostate cancer, the status of a candidate tumor suppressor
gene named PRLTS gene, recently cloned from the same region in
some human malignancies, was examined in the present study. DNAs
were isolated from 69 Japanese prostate cancer patients (37 localized
and 32 cancer- death cases). Loss of heterozygosity at this gene
locus was observed in 15 of 36 (42%) localized prostate cancer
patients and 22 of 32 (69%) cancer-death patients. One cancer-death
patient had a missense mutation, ACG-->ATG (Thr-->Met) at codon
64 in metastatic tumor tissues of pelvic lymph node and liver,
and these tissues showed loss of the homologous allele, indicating
that "two-hit" mutation of the PRLTS gene had occurred in this
case. The others did not show any mutation, regardless of the
presence or absence of loss of heterozygosity. Although loss of
heterozygosity at the PRLTS gene locus is a relatively common
abnormality, mutation of this gene is rare in prostate cancer.