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11
Author: Suzuki, H.; Komiya, A.; Aida, S.; Ito, H.; Yatani, R.; Shimazaki,
J.
Year: 1996
Title: Detection of human papillomavirus DNA and p53 gene mutations
in human prostate cancer
Journal: Prostate
Volume: 28
Issue: 5
Pages: 318-24
Label: 96201735
Keywords: Adenocarcinoma/*genetics/*virology
Base Sequence
DNA, Viral/*isolation & purification
Genes, p53/*genetics
Human
Male
Molecular Sequence Data
*Mutation
Papillomavirus, Human/*genetics
Polymerase Chain Reaction/methods
Polymorphism, Single-Stranded Conformational
Prostatic Neoplasms/*genetics/*virology
Support, Non-U.S. Gov't
Abstract: The relationship between integration with human papillomavirus
(HPV) and p53 gene mutations in tissues of prostate cancer were
examined. Tissue samples analyzed were obtained by total prostatectomy
(29 stage B cancer cases) and from autopsy (22 endocrine therapy-resistant
metastatic disease cases). HPV DNA was detected in 8 of 51 (16%,
5 in stage B and 3 in autopsy cases) by polymerase chain reaction
(PCR) using consensus primers on L1 region. Genotypes of HPV were
entirely type 16. Structural abnormalities of p53 gene were detected
in 7 of the 22 autopsy cases (32%) by PCR-single-strand conformation
polymorphism analysis and direct sequencing. No p53 gene mutation
was found in stage B cancer cases. Analysis of mutation spectra
revealed clear differences between Japanese and Westerners. There
was a significant difference in the mutation frequency between
stage B and autopsy cases (p 0.01, Fisher's exact test). One case
showed both integration of HPV and p53 gene mutation in different
cancer foci. However, the other cases revealed an inverse correlation
between the presence of HPV DNA and p53 gene mutations. These
data show that p53 genetic alteration is correlated with the progression
of prostate cancer, in contrast to the integration of HPV that
may occur in a relatively early stage. In conclusion, this study
may indicate that either p53 gene mutation or the presence of
HPV's oncogenic protein E6 is involved in the development of prostate
cancer.