プライマーリスト

　 Name Sequence (5' to 3') Tm (｡C) G/C(%) bp Appendix
for RT-PCR 　 　 　 　 　 　 　 SL1-22 GGTTTAATTACCCAAGTTTGAG 52 36.36 22 　
SL1-20 GGTTTAATTACCCAAGTTTG 48 35 20 　
SL1-20Pst acctgcaGGTTTAATTACCCAAGTTTG 52 40 28 　
SL2-22 GGTTTTAACCCAGTTACTCAAG 54 40.91 22 　
SL2-20 GGTTTTAACCCAGTTACTCA 50 40 20 　
5'RACE Anchor GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG 67 63 36 MluI, SalI & SpeI sites
3'RACE Anchor GGCCACGCGTCGACTACTTTTTTTTTTTTTTTTT 59 35.29 34 MluI & SalI sites
Universal for RACE GGCCACGCGTCGACTAC 53 70.59 17 MluI & SalI sites
for 2-hybrid vectors 　 　 pGAD-5' TTCGATGATGAAGATACC 45 38.89 18 for pGAD-Cx, pACT, pACT2 vector
pGBD/GBDU-5' AAGAGAGTAGTAACAAAG 43 33.33 18 for pGBD/pGBDU-Cx vector
pGXD-3' TGAAGTGAACTTGCGGGG 55 55.56 18 for pGAD/pGBD/pGBDU-Cx, pACT
for pPD vectors 　 　 PPD-NLS CTTCTTCTTTGGAGCAGTCA 58 45 20 locates on NLS of pPD vectors
GFP-pPD CCCATTAACATCACCATCTA 54 39.13 20 locates on 5' of gfp gene
RFP-pPD CGGTGTTGTGGCCCTCGTAG 63.9 65 20 locates on 5' of rfp gene
universal primers 　 　 　 　 　 　 　 　 SK20 CGCTCTAGAACTAGTGGATC 54 50 20 for pBluescript vectors, XbaI & SpeI sites
SK TCTAGAACTAGTGGATC 43 41.18 17 for pBluescript vectors, XbaI & SpeI sites
KS20 CCTCGAGGTCGACGGTATCG 61 65 20 for pBluescript vectors, XhoI & SalI sites
KS TCGAGGTCGACGGTATC 49 58.82 17 for pBluescript vectors, SalI site
T7 GTAATACGACTCACTATAGGGC 56 45.45 22 for pBluescript vectors only , NOT for pET vectors
T3 AATTAACCCTCACTAAAGGG 　 　 20 　
M13RV GGAAACAGCTATGACCATG 51 47.37 19 for pUC derived vectors
M13(-21) TGTAAAACGACGGCCAGT 　 　 18 　
M13(-185)

TAAGGAGgAAATACCGCATCAGGC
(wrong SEQ, but work)

TAAGGAGAAAATACCGCATCAGGC
(correct SEQ)

59 50 24 paried with TM-9B, 185bp downstream from MCS
for pET vectors T7pro_pET TAATACGACTCACTATAGGG 50 40 20 　
T7ter_pET GCTAGTTATTGCTCAGCGG 57 52.63 19 　
S-tag(pET43.1) GAACGCCAGCACATGGAC 59.9 61.1 18 　

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