発現ベクター
ベクターの名前をクリックすると配列が見れます。

name size
(bp)		 tag promotor host selection seq.
primer		 flame origin MCS ref.
pBlueScript II SK(-) 2958 b-gal* T7 JM109 Amp T7
primer		 one this
lab.		 BamHI, PstI, EcoRI, EcoRV, HindIII, SalI, XhoI, etc. TOYOBO
pET16b 5711 His (10 aa) T7 BL21
(DE3)		 Amp - one Novagen NdeI, XhoI, BamHI Novagen
pET28a(+)
pET28b(+)
pET28c(+)		 5369 His (6 aa)
+ T7 tag		 T7 BL21
(DE3)		 Km - three Novagen BamHI, EcoRI, SacI, SalI, HindIII, NotI, XhoI Novagen
pET32a(+)
pET32b(+)
pET32c(+)		 5900 Trx tag/
His(6 aa)
+ S tag		 T7 BL21
(DE3)		 Amp - three Chronos
lab.		 NcoI, EcoRV, BamHI, EcoRI, SacI, SalI, HindIII, NotI, XhoI Novagen
pMAL-c2* 6646 MBP* tac JM109 Amp - one Dr.
Qadota		 XmaI, EcoRI, BamHI, XbaI, SalI, PstI, HindIII NEB
pMAL-p2 6721 MBP* tac JM109 Amp - one Dr.
Qadota		 同上 NEB
pMAL-KK-1*
pMAL-KK-2*
pMAL-KK-3*		 6754
6755
6756		 MBP* tac JM109 Amp - three Dr.
Qadota
modified		 BamHI, EcoRI, SmaI, SalI, XhoI, ClaI, HindIII, XbaI -
pGEX-4T-1
pGEX-4T-2
pGEX-4T-3		 4970
4969
4968		 GST* tac JM109 Amp - three Dr.
Qadota		 BamHI, EcoRI, SmaI, SalI, XhoI, NotI TaKaRa
pGEX-KK-1
pGEX-KK-2
pGEX-KK-3		 4900
+a		 GST* tac JM109 Amp - three Dr.
Qadota
modified		 BamHI, EcoRI, SmaI, SalI, XhoI, ClaI, HindIII, XbaI TaKaRa
pUR278
pUR288
pUR289		 5351
5346
5347		 b-gal* lac JM109 Amp - three this
lab.		 BamHI, SalI, XbaI, HindIII, ClaI Molecular
Cloning
pUR290*
pUR291*
pUR292*		 5353
5348
5349		 b-gal* lac JM109 Amp - three this
lab.		 同上 Molecular
Cloning
pHK1* 5.8 b-gal* lac JM109 Amp HK2
HK3		 one Dr.
Kagawa
modified		 EcoRI, SmaI, BamHI, SalI, PstI Molecular
Cloning

*pMAL-c2
*pMAL-KK-1-3
*pUR290-292
*pHK1
*b-gal
*MBP
*GST		 ：cytoplasmic expression (malE signal seq. deletion)
：Backbone is pMAL-p2
：vector unstable
：Back bone is pEX2
：b-galactosidase
：maltose binding protein
：glutathione S-transferase

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