| 1M Tris-HCl (pH 8.0) |
0.5 ml |
|
| 100 mM EDTA (pH 8.0) |
0.5 ml |
|
| D.D.W. |
up to 50.0 ml |
then sterilize |
| 1M NaCl |
10.0 ml |
|
| 1M Tris-HCl (pH 8.0) |
1.0 ml |
|
| 100 mM EDTA (pH 8.0) |
1.0 ml |
|
| D.D.W. |
up to 100.0 ml |
then sterilize |
| 1M Tris-HCl (pH 8.0) |
2.5 ml |
|
| 1M EDTA |
1.0 ml |
|
| D.D.W. |
up to 95.0 ml |
|
| オートクレーブ後、1M Glucose (sterilized)を5ml加える。 | ||
| 2N NaOH |
1.0 ml |
|
| 10% SDS |
1.0 ml |
0.45nmメンブレンで濾過 |
| D.D.W. |
up to 10.0 ml |
| 5M potassium acetate |
60.0 ml |
|
| glacial acetic acid |
11.5 ml |
|
| D.D.W. |
28.5 ml |
文責：T