University of Vermont から得たハムスターについての情報
■Blood Collection Site
Heart あるいは orbital sinus （眼窩静脈洞）で
Heart puncture should be performed only on animals under anesthesia. It is recommended only as a terminal procedure.
These techniques may require anesthesia under procedures established by the Institutional Animal Care and Use Committee.
麻酔下で、更に最終的な手段で行うべきとの注意がある。
眼窩穿刺はかわいそうなので Saphenous vein puncture for blood sampling of the hamster. 伏在静脈の穿刺による方法がBlood Collection in mouse from saphenous vein?に画像入りで詳しく説明されています。その方法は
■Blood Injection Site
No practical IV sites とあり。特定の場所はないらしい。
■Recommended Temperatures
室温は 18-26℃ が奨励されている。
■Agents and Methods of Euthanasia by Species
Acceptable
Barbiturates, Inhalant anesthetics, CO, CO2, microwave irradiation, Potassium chloride in conjunction with general anesthesia
Conditionally acceptable
N2, Ar, cervical dislocation (rats <200 g), decapitation
■Needle Sizes and Recommended Injection Volumes
Intravenous 静脈注射
Femoral or jugular vein (cut down), 0.3 ml, < 25 gauge 大腿静脈 、頸静脈
lntraperitoneal 腹腔内
3-4 ml, < 21 gauge posterior
Intramuscular 筋肉内
Quadriceps/ posterior thigh, 0.1 ml, < 21 gauge 大腿四頭筋、後肢
Subcutaneous 皮下
Scruff, 3-4 ml,< 20 gauge 首筋、うなじ
■Routes of drug administration in small mammals
Intramuscular
Gluteal 0.10ml <21G 殿部
Subcutaneous
Scruff 3.00-4.00 ml <20G
Intraperitoneal
10 degree angle to Abdominal wall 3.00-4.00 ml <21G
Intravenous
Femoral or jugular vein (cut down) 0.30 ml <25G
Oral
0.75 ml Bulb needle
Maximum volumes are given for administration by the im, sc, ip and iv routes. Larger volumes than those indicated may be given by the oral route. However, those given are sufficient and safe for most purposes
■Physiological and Other Data
TEMPERATURE 36.1-38.9
RESPIRATORY B/M 30-140
RESPIRATORY MINUTE VOL. l/ .06
RESPIRATORY TIDAL VOLUME m .4-1.4
PaCO2 mmHg 59
OXYGEN USE ML/G/HR .6-1.4
HEART RATE B/M 250-500
ARTERIAL BLOOD PRESSURE mm 150/100
ARTERIAL BLOOD pH 7.39
BLOOD VOLUME ml/kg TOTAL 78
BLOOD VOLUME ml/kg EXSANGU 30 全血を採る
BLOOD VOLUME ml/kg 1 SAMP 5
BLOOD CALCIUM mEq/L -mg/100 5-12mg/
BLOOD PHOSPHORUS mg/100ml 3.4-8.2
BLOOD SODIUM mEq/L 144
BLOOD CHLORIDE mEq/L 106
BLOOD POTASSIUM mEq/L 4.72
BLOOD MAGNESIUM mg/100ml 2.5
BLOOD CHOLESTEROL mg/100ml 25-135
BLOOD SERUM PROTEIN gm/100m 4.5-7.5
BLOOD ALBUMIN gm/100ml 2.6-4.1
BLOOD GLOBULIN gm/100ml 2.7-4.2
BLOOD GLUCOSE - mg/100ml 60-150
BLOOD UREA NITROGEN MG/DL 12-25
CREATININE MG/DL .91-.99
TOTAL BILIRUBIN MG/DL .25-.6
ALK.PHOSPHATASE i.u./L 54.60
BLOOD HEMATOCRIT % 36-59
BLOOD HEMOGLOBIN g/100ml 2-30 (10-16)
BLOOD RBC 10-6/mm3 4-10
BLOOD PLATELETS 10-3/mm3 160-516
BLOOD LEUCOCYTES 10-3/mm3 3-11%
BLOOD NEUTROPHILS 10-3/m 10-42%
BLOOD EOSINOPHILS 10-3/ 0-4.5%
BLOOD BASOPHILS 10-3/ 0-1%
BLOOD LYMPHOCYTES 10-3/ 50-95%
BLOOD MONOCYTES 10-3/ 0-3%
BLOOD COLLECTION SITE HRT, ORB.SIN.
BLOOD INJECTION SITE NONE
FEED DAILY gm/kg >15g/100g
WATER >20ml/100
ROOM TEMPERATURE C 18-26 (71F)
ROOM HUMIDITY % 45-55
ROOM LIGHT HOURS/DAY 10-12
LITTER MATERIAL SPECIAL
WEIGHT ADULT MALE gm 80-130
WEIGHT ADULT FEMALE gm 80-150
WEIGHT AT BIRTH gm 2
BREEDING AGE-MALE DAYS 10-14 WKS
BREADING AGE-FEMALE DAYS 6-10 WKS
ESTRUS CYCLE DAYS 4-15
GESTATION DAYS 15-19
WEANING AGE DAYS 20-25
LITTER SIZE 5-10
BREEDING AFTER PARTURITION 4-6 days
BREEDING LIFE-MALE YEARS 1
BREEDING LIFE-FEMALE YEARS 1 (5-7 LITTERS)
BREEDING/MATING DATA MALE 1/1
MILK FAT % 12
MILK LACTOSE % 3.4
MILK PROTEIN % 9
BODY SURFACE AREA CM2 10.5
DIPLOID NUMBER KARYOTYPE 44
LIFE SPAN YEARS 1.5-2
CAGE SIZE LXWXH 19SIX6