铜脱颁帕灰のコムギゲノムへの瞥掐

甫垫亨瘟

Wheat, rye, Thinopyrum intermedium, Dasypyrum villosum and other wheat relatives.

Th. intermedium , wheat	and D. villosum

誊弄

The wild relatives of wheat are important genetic resources for wheat improvement, in particular, in the aspect of introducing disease resistance into common wheat. Up to now, many wheat varieties with alien disease resistant genes have been developed and taken an important role in wheat productivity. In order to utilize these alien genes more effectively, three main alien species of wheat including S. cereale, D. villosum, and Th. intermedium are used to synthesize trigeneric and tetrageneric hybrids in wheat genetic background. Multi-alien genomes with different powdery mildew resistant genes are combined to obtain multiple disease resistance. In addition, new repetitive DNA sequences are isolated from D. villosum and applied to identify individual D. villosum chromosomes.

1. Production of trigeneric and tetrageneric hybrids involving Triticum, Secale, Dasypyrum and Thinopyrum to demonstrate the evolution relationship and genomic rearrangement.

2. Identification of alien chromosomes in the trigeneric hybrids or alien chromosome segments with useful genes in aneuploids by means of molecular cytogenetics method.

3. Multiple disease resistance, multi-alien genomes carrying different powdery mildew resistance from rye and D. villosum are desirable to be introduced into the wheat genomes.

4. Isolation and application of new repetitive DNA sequence from D. villosum. Meanwhile, we will present the in situ hybridization patterns of the new sequence and its use in detecting D. villosum chromosome segments in wheat genome.

In this study, molecular biology and molecular cytogenetics method are used to present the evidence of alien DNA or genomes in wheat genetic background. Especially, multi-color FISH method is used to detect the different alien chromosomes simultaneously.

甫垫の萎れ

▲ Procedure for DNA Cloning

甫垫数恕

1. DNA Cloning and sequencing

2. Southern blot hybridization

3. Sequential C-banding and fluorescence in situ hybridization

4. RT-PCR for differential display